THE DETERMINATION OF DIKETO-l-GULONIC ACID, DEHYDRO-l-ASCORBIC ACID, AND l-ASCORBIC ACID IN THE SAME TISSUE EXTRACT BY THE 2,4-DINITROPHENYLHYDRAZINE METHOD
نویسندگان
چکیده
منابع مشابه
Metabolism of l-ascorbic acid, dehydro-l'ascorbic acid, and diketo-l-gulonic acid in the guinea pig.
The interrelationships of L-ascorbic acid (AsA) and dehydro-L-ascorbic acid (DHA) have been investigated both in viva and in vitro by numerous workers, with especial emphasis upon the conversion of DHA to AsA by the animal body (l-8). The conversion of DHA to diketo-L-gulonic acid (DKA) in vitro has been shown by Herbert and coworkers (3), by Borsook (l), and by Penney and Zilva (6), and this c...
متن کاملPossible Formation of Dehydro-L-ascorbic Acid from 2,3-Diketo-L-gulonic Acid in an Aqueous Solution.
The reaction of 2,3-diketo-L-gulonic acid (DKG), which is one of the important intermediate products in the degradation of L-ascorbic acid (ASA) in both food and biological systems, in an aqueous solution was studied. The formation of a small amount of the γ-lactone, dehydro-L-ascorbic acid (DASA), from DKG was observed. This strongly suggests the chemical possibility of a reverse reaction in D...
متن کاملDetermination of L- Ascorbic Acid in Plasma by Voltammetric Method
Voltammetric techniques have been considered as important methods among the analytical techniques used for the identification and determination of trace concentrations of many biological molecules such as L-ascorbic acid (AA). L-ascorbic acid is an electro-active molecule, though it is difficult to determine its value directly with a majority of electrodes made of carbon and transition metals, ...
متن کاملDetermination of L- Ascorbic Acid in Plasma by Voltammetric Method
Voltammetric techniques have been considered as important methods among the analytical techniques used for the identification and determination of trace concentrations of many biological molecules such as L-ascorbic acid (AA). L-ascorbic acid is an electro-active molecule, though it is difficult to determine its value directly with a majority of electrodes made of carbon and transition metals, ...
متن کاملL-Ascorbic acid degradation by bacteria. VIII. All the pathway in the L-ascorbic acid metabolism.
The medium used contained 3g of substrate, 150ml of 1/15M phosphate buffer and 150ml of sterile distilled water. 0.5g (dry weight) of the adapted cells was added to the medium and the whole was incubated at 30•‹ for 20 hours. For detecting the intermediate products, paper chromatography was chiefly used. For developing the nonvolatile acids, n-butanol-acetic acid-water (4:1:1) was used and the ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1948
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)57387-7